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Abdalla ELMILEIK1 , Naoki ISOBE1 , Takato TERADA1
Hiroshima University, Japan 1
This study examined the in vitro development of bovine oocytes following addition of bovine follicular fluid (bFF) from large healthy follicles (?15 mm) to maturation medium from the start of culture (i.e. GV), at the presumed timing of completion of GVBD or at the completion of the first meiosis. The oocytes were matured in the basic medium (TCM199+estrous cow serum(ECS)+LH+FSH: BM) or in BM with various conc. of bFF (15, 30, 50, 70 or 100%) added at 0 h, 8 h or 18 h from the start of culture. After 24 h, the oocytes were inseminated with frozen-thawed caffeine treated semen (final conc.: 5 x 106/ml). After 7 h, the oocytes were cultured in TCM199+10% ECS for 10 days. On day 7, some blastocysts from the bFF- and bFF-free were stained with propidium iodide and examined under lazer scanning microscope: significantly (P<0.05) more cells in bFF embryos, 76 vs 38/embryo. Addition of bFF (15%) at 0 h or 8 h was significantly (P<0.05) beneficial for development to blastocysts (38, 39% vs 25% in bFF-free), whereas conc. higher than 15% were detrimental or without effect (22% blastocysts in 30% and 14% in 50%). However, higher conc. were similarly beneficial when the oocytes were transferred into them after 18 h of culture in the bFF-free maturation medium, and the effect was maximum at 50% concentration of bFF (blastocysts: 38%). The results suggest that bFF contains substance(s) which (at least in part) supports cytoplasmic rather than nuclear maturation since the effect on development was best when the oocytes were placed in the bFF medium after 18 h of culture in bFF-free medium.
This abstract is being presented on Monday, August 2 at 8:00 AM to 10:15 AM at CUB 2nd Floor Ballroom.