|Back Topic Categories Search Previous Abstract Next Abstract|
Ali Ahmady1 *, Soon-Chye Ng1 *
Department Obstetrics & Gynaecology, National University of Singapore, Singapore, Singapore 1
Two main structural deficiencies occur in the infertile men's ejaculate resulting in sperm death: primary necrosis due to infectious and consequent inflammation, and apoptosis due to anatomical or hormonal disorder. The aim of this investigation is to study the developmental capacity of sperm at various levels of death. The sperm were subjected to various treatments to induce death at the different levels (cellular and molecular) prior to injection into the ooplasm. We demonstrate that with dead sperm, the key point in the fertilization process is the activation of the oocyte, by injection of cytosolic sperm factor in our experiments. Fertilisation rate was 17.5% when dead sperm were injected into the oocyte, compared to 55.0% in control group (live sperm). The fertilisation rate was increased to 62.8% in
This abstract is being presented on Sunday, August 1 at 8:00 AM to 10:15 AM at CUB 2nd Floor Ballroom.