Abstract: 466

EVIDENCE ON THE ASYMMETRIC ABILITY IN THE ESTROGEN SECRETION BY THE RAT OVARIES.

R Domínguez^{1 *}, AI Barco¹ , R Chavira¹ , ME Cruz¹
Biology of Reproduction Research Unit, FES Zaragoza UNAM, México D.F., México ¹

The possible existence of asymmetrical ovarian involvement in the estrogen secretion was examined. On the day of estrus of 4-day cyclic rats, after two consecutive estrous cycles, sham operation or hemiovariectomy (left or right ovary extirpated) was performed at 1200 of the day of estrous and the animals were killed 1 or 2 hours after surgery. Untouched control groups were sacrificed at 1300 or 1400 h. One hour after sham operation, progesterone (22.2 ± 4.3 vs 23.8 ± 4.6 ng/ml) and 17-estradiol (19.3 ± 2.8 vs 16.3 ± 3.0 pg/ml) serum levels, were similar to untouched group. Rats with left ovary in situ showed a diminution in 17-estradiol serum level (19.3 ± 2.8 vs 9.5 ± 1.8, p<0.05) without changes in progesterone level (22.2 ± 4.3 vs 25.1 ± 4.6). When the right ovary was in situ, 17-estradiol (17.9 ± 2.6 vs 8.5 ±1.3, p<0.05) and progesterone (21.0 ± 3.0 vs 11.7 ±3.0, p<0.05) serum levels decreased, in comparison with sham operated animals. Two hours after sham operation, progesterone (16.0 ± 4.0 vs17.8 ±3.5) and 17-estradiol (11.3 ±1.4 vs 10.1 ± 2.0) serum levels were similar to untouched control group. 17-estradiol (11.3 ± 1.4 vs 12.9 ± 2.6) and progesterone (16.0 ±4.0 vs 22.2 ±3.5) serum level were similar to sham operated animals when the rats kept the left ovary in situ. When the right ovary was in situ, the 17-estradiol serum levels was lower than in sham operated ones (6.3 ± 0.4 vs 14.9 ±2.3, p<0.05), while progesterone (37.4 ±11.2 vs 12.4 ± 2.3, p<0.05) serum levels increased. These results suggest that ovarian steroidogenesis ability by the left and right ovaries is different when one ovary is extirpated. Supported by DGAPA:IN 208996 , CONACYT and PUIS.

    This abstract is being presented on Tuesday, August 3 at 8:00 AM to 10:15 AM at CUB 2nd Floor Ballroom.