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Valerie Latimer1 , Vasilios Garyfallou1 , Steve Kohama1 , Henryk Urbanski1
Division of Neuroscience, Oregon Regional Primate Research Center, Beaverton, OR. 1
It has recently been shown that a second form of gonadotropin-releasing hormone (GnRH-II) exists in the primate brain and is capable of stimulating luteinizing hormone release. However, little is known about the distribution of GnRH-II mRNA and the control of its expression during developmental events, such as puberty. Therefore, the aim of this study was to examine GnRH-II mRNA in the hypothalami of pre-pubertal (7 months) and adult (10 - 15 years) male and female rhesus macaques (Macaca mulatta, n=3/group). Eight hypothalamic sections (25 Ám) per animal were processed for in situ hybridization using a 35S-labeled, anti-sense, GnRH-II riboprobe. The pre-pubertal animals showed a high level of expression of GnRH-II mRNA in the supraoptic and paraventricular nuclei, and to a lesser extent in the suprachiasmatic nucleus. The adults showed a similar mRNA distribution pattern, but also expressed GnRH-II in the medial basal hypothalamus (MBH). These results imply that the MBH upregulates GnRH-II expression during development and may represent a mechanism that controls the onset of puberty in primates. Grant Support: NIH HD-29186 and RR-00163
This abstract is being presented on Sunday, August 1 at 8:00 AM to 10:15 AM at CUB 2nd Floor Ballroom.