Submission Number: ROB-4-20-4

Abstract Number: 548

EFFECTS OF EARLY POSTNATAL ANDROGENIZATION OR ESTROGENIZATION ON THE MONOAMINES IN THE ANTERIOR (AH) AND MEDIUM HYPOTHALAMUS (MH) OF THE FEMALE RAT.

Maria Elena Ayala* ¹, Sonia Ruiz ¹, Maria de Jesus Moran ¹, Antonin Bukovsky ² and Roberto Dominguez* ¹

Biology of Reproduction Research Unit. FES Zaragoza UNAM. México D.F. México ¹
University of Tennessee Graduate School of Medicine, Knoxville, TN, USA. ²

Abstract:
Single dose of testosterone propionate (TP) or estradiol dipropionate (ED) results in the development polycystic ovaries. There is an evidence on the role of monoamines in the regulation of gonadotropin secretion. Noradrenaline (NA), dopamine (DA) and serotonin (5-HT) were quantified by HPLC in the AH and MH of rats treated with 500 mg of TP, 100 mg of ED, or oil vehicle on postnatal day 5. Animals were sacrificed at two months of age. The concentration of NA was increased in the AH and MH (AH: 4.70.7 g/mg vs. 2.60.3; MH: 5.01.1 vs. 2.70.5, p<0.05) in animals treated with TP, but concentration of DA was lower in the AH (0.140.03 /mg; 0.170.05 vs. 0.240.05, p<0.05). Serotonin levels in AH and MH were not modified in TP rats, and no significant changes were detected in ED females. These results suggest that: (1) TP induces changes in the hypothalamic catecholamines, and (2) anovulation after ED has a different mechanism when compared to the TP females. Additional studies (2 months after ovariectomy) are required to determine if the observed changes are not influenced by an altered steroidogenesis from the ovaries. Supported by DGPA IN-208796. .

Keywords: hypothalamus, monoamines, androgenization, newborn rat

This abstract is being presented at: 8:00 AM in session:
Neuroendocrinology II