Submission Number: WEI-4-16-2

Abstract Number: 494

A STUDY ON LOCALIZATION OF 5-HT AND ITS RECEPTORS IN HUMAN PLACENTA.

Bing Yao ¹ and Wei-Quan Huang ²

Institute of Animal Research, Chinese Academy, Beijing, China ¹
Dept of Histology and Embryology, The Fourth Military Medical University, Xi'an 710032, China ²

Abstract:
The localization of 5-hydroxytryptamine (5-HT) and its receptors in human placentas was studied at light and electron microscopic levels by using immunohistochemistry and in situ hybridization. The syncytiotrophoblast, cytotrophoblast, stromal cells and decidua cells in human placentas were all appeared 5-HT immunoreactivity. The 5-HT immunoreactive material was distributed in cytoplasm with negative nuclei. The 5-HT immunoreactive material was was also contained in capillary endothelium. The culture trophoblast cells in medium free of serum were also shown 5-HT immunoreactivity in cytoplasm. Both the syncytiotrophoblast and cytotrophoblast in human placentas and fetal white blood cells were not only appeared 5-HT receptor immunoreactivity but also were detected 5-HT1A receptor mRNA hybridized signal in cytoplasm. The stromal cells and capillary endothelium in placental villi and maternal decidua cells were all shown 5-HT receptor immunoreactivity in cytoplasm. By immunohistochemistry at electron microscopic level, the small flattened vesicles and large dense cored vesicle within trophoblast cells were shown 5-HT receptor immunoreactivity. These results suggested that human placenta may not only produce 5-HT but also be a 5-HT target organ, its producing 5-HT may not only play roles in placenta development and pregnancy maintain by paracrine and autocrine but also take part in regulating fetal development by endocrine mechanisms. .

Keywords: 5-HT, Receptor, Placenta, Human

This abstract is being presented at: 8:00 AM in session:
Hormone Receptors