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BASIGIN EXPRESSION AND HORMONAL REGULATION IN MOUSE UTERUS AND EMBRYOS DURING THE PERI-IMPLANTATION PERIOD.
Xiao, Li-Juan1, Chang, Hong1, Ding, Nai-Zheng1, Kadomatsu, Kenji2, Yang, Zeng-Ming1, 1 2
ABSTRACT- Basigin, a transmembrane glycoprotein belonging to the immunoglobulin superfamily, has been shown to be essential for implantation and fertilization. The aim of this study was to determine basigin expression and hormonal regulation in mouse uterus and embryos during peri-implantation using in situ hybridization, immunohistochemistry and reverse transcription-PCR. Basigin immunostaining and mRNA were mainly localized in the luminal and glandular epithelium on day 1 of pregnancy and gradually decreased to a basal level from days 1 to 4 of pregnancy. On day 5 of pregnancy, basigin expression was only detected in the implanting embryos, and the luminal epithelium and sub-luminal stroma immediately surrounding the embryos. A similar expression pattern of basigin was also induced in the delayed-implantation uterus activated by estrogen injection. On days 6 to 8 of pregnancy, basigin expression was detected in the embryos and secondary decidua. Estrogen significantly stimulated basigin expression in the ovariectomized mouse uterus. A high level of basigin immunostaining and mRNA was also seen in proestrous and estrous uteri. By reverse transcription-PCR analysis, basigin expression was detected in every stage of mouse embryos during the preimplantation development. Basigin level was high in oocytes and zygotes, was low at 2-cell stage, began to increase from 4-cell stage and reached a high level at the blastocyst stage. These results suggest that basigin is closely related to mouse implantation and up-regulated by estrogen.
KEY WORDS: mouse, basigin, uterus, implantation
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