PARENT SESSION
Fertility Regulation
367
THE ROLE OF FERTILIZATION-PROMOTING PEPTIDE ON ACROSOME REACTION AND GLYCOSIDASE ACTIVITY IN VITRO OF FROZEN-THAWED SPERMATOZOA IN PIG.
Park, Choon-Keun1, Hwang, In-Seon1, Cheong, Hee-Tae1, Yang, Boo-Keun1, Lee, Jang-Hee2, Kim, In-Cheul2, Kim, Choung-Ik1, 1 2
ABSTRACT- This study evaluated the effect of fertilization-promoting peptide (FPP) on glycosidase activity of spermatozoa frozen-thawed in pig. Using chlortetracycline (CTC) fluorescence, the various glycosidase inhibitors have been assessed for acrosome reaction, and the oocyte penetration test to determine whether the FPP can promote the fertilizing ability. When frozen-thawed spermatozoa was washed with different concentrations of FPP, there were significantly (P<0.05) more acrosome-reacted in medium with 100 nM than 0, 50, 200 and 400 nM. The penetration rates were also higher in medium containing with 100 nM FPP (P<0.05). In spermatozoa washed with different concentrations of FPP, acetylglucosaminidase activity was at least twofold higher than fucosidase, mannosidase and galactosidase. In same glycosidase, however, there were no difference in medium with different concentrations of FPP. In another experiment, spermatozoa preincubated in medium with or without FPP (100 nM) for 0, 1, 2, 3 and 4 h were inseminated with oocytes matured in vitro. The percentages of spermatozoa that reached acrosome reaction were affected by preincubation and were higher in medium with that than without FPP. When oocytes were inseminated with spermatozoa preincubated in medium with and without FPP during the different periods, penetration rates were decreased with preincubation periods of spermatozoa. However, the penetration rates were higher in spermatozoa preincubated with than than without FPP. The activities of fucosidase, mannosidase, galactosidase and glucosaminidase were also higher in medium with than without FPP. These results suggest that FPP may play a positive role in promoting of sperm function and glycosidase activity in pig. This work was supported by Korea Research Foundation Grant (KRF-99-041-G00056 G4103).
KEY WORDS: fertilization-promoting peptide, acrosome reaction, glycosidase activity, pig