|HOME SCHEDULE AUTHOR INDEX SUBJECT INDEX|
THE INVOLVEMENT OF ACTIVIN AND TGF1 IN THE REGULATION OF TELEOST OVARIAN STEROID PRODUCTION.
Calp, Katie1, Van Der Kraak, Glen1, 1
ABSTRACT- Local ovarian factors have been recognized for their contribution to the regulation of vertebrate ovarian function. Activin and TGF1, members of the transforming growth factor (TGF) superfamily of polypeptide growth factors, are produced in the mammalian ovary and have been shown to exert their local effects via autocrine/paracrine mechanisms. Activin and its receptors have been cloned in the teleost ovary, and have been shown to exert effects on oocyte maturation. The effects of goldfish recombinant (gr) activin-B, human recombinant (hr) activin-A and hr TGF1 on teleost ovarian steroidogenesis were investigated using an in vitro incubation system. Vitellogenic or preovulatory full grown (PFG) goldfish follicles were incubated with gr activin-B (5.0 IU/mL), hr activin-A (50 ng/mL) or hr TGF1 (50 ng/mL) alone, or in the presence of human chorionic gonadotropin (hCG; 1.0 or 10.0 IU/mL). Steroid hormone levels were measured in the incubation medium by RIA. In PFG follicles, both activin-A and TGF1 significantly decreased gonadotropin (GtH)-stimulated production of 17,20-dihydroxy-4-pregnen-3-one, which is the maturation-inducing hormone in this teleost fish species. Activin-A also significantly decreased GtH-stimulated testosterone production in these follicles. Neither activin-A nor TGF1 affected GtH-stimulated estradiol production from vitellogenic follicles. This research demonstrates that members of the TGF superfamily are active in the goldfish ovary, exerting differential effects on ovarian steroid production that appear to be related to the stage of ovarian maturity. Members of the TGF superfamily may play an important role in the regulation of teleost ovarian function.
KEY WORDS: activin, TGF1, teleost, steroidogenesis
Internet Services provided by|
Allen Press, Inc. | 810 E. 10th St. | Lawrence, Kansas 66044 USA
e-mail firstname.lastname@example.org | Web www.allenpress.com
All material is copyright © 2001 SSR