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(569) EFFECT OF ACID PHOSPHATASE ON BOVINE OVIDUCT EPITHELIAL CELL CULTURED WITH AND WITHOUT FETAL CALF SERUM. Goncalves, Roseli1,2, D'Angelo, Magali2, Ferreira, Nilson1, 1 Anatomia dos Animais Domesticos, Sao Paulo, SP, Brasil2 Centro de Sanidade Animal, Sao Paulo, SP, Brasil ABSTRACT- The oviduct is the physiological site for some events in reproduction, such as capacitation of espermatozoa, fertilization and early embryonic development. Interactions between oviduct epithelial cells and gametes or embryos cannot sufficiently be studied in vivo. Therefore, model systems are needed which mimic in vivo conditions most closely. Use of serum may introduce toxic components or pathogens such as viruses or the spongiform encephalopathy prion. Serum contains a wide variety of unidentified components , possibly including fatty acids, growth factors, amino acids, and/or vitamins. The aim of the present study was to examine tha acid phosphatase activity in primary culture with or without serum, because is a sensitive method that permit to determine the early modifications events and injury of the cellular functions. The method for isolating bovine oviduct epithelial cells (BOEC) was the same described for EYESTONE and FIRST, 1989. The cells were cultured in TCM 199 (Gibco) supplemented or not with 10% fetal calf serum (FCS), pyruvate, penicillim, streptomycin at 39°C and CO2 in air. The cultured supernatant of different days was analyzed with Doles kit and observed in spectrophotometer U2001 Hitachi, 410nm. Both cultures presented two cell populations: ciliary and secretory that gradually dedifferentiate during culture. These cultured with FCS formed a confluent monolayer after 5days, but in serum-free media they haven't formed a complete monolayer. Total of acid phosphatase content in the culture supernatant had an increased in both cultures until the day 10. Our data show that BOEC cultured under serum-free conditions have presented no significant differences in acid phosphatase activity in both cultures and perhaps may be used as a model system to study the interactions between bovine oviduct cells and gametes or embryos. Financial supported by FAPESP fellowship#98/07813-5. KEY WORDS: bovine oviduct, acid phosphatase, culture, fetal calf serum |
