PARENT SESSION
TESTICULAR SPERMATOGENESIS, MORPHOGENESIS, AND GENE EXPRESSION
Kent
7:30 AM-10:00 AM

(470) COMPARATIVE SEMEN MOTION ANALYSIS BY TWO COMPUTER-ASSISTED METHODS IN AI BULLS.

Gábor, György¹, Nagy, Szabolcs², Szász, Ferenc³, Szigeti, Edit⁴, Solymosi, Norbert⁵, ¹ Dept of Cattle Breeding, Herceghalom, Hungary² Dept of Cell Biology, Herceghalom, Hungary³ Androvet Ltd., Budapest, Hungary⁴ Research Group for Animal Breeding of the Hungarian Academy of Science, Godollo, Hungary⁵ Central Veterinary Institute, Budapest, Hungary

ABSTRACT- Examination of semen motility by computer assisted methods have been developed during the last 20 years. The aim of this study was to compare a currently self-developed computer assisted semen analyzing method (CASPAR, Pictron Ltd, Budapest, Hungary) with one of the most widely used system (SM-CMA, MTG GmbH, Altdorf, Germany). Semen samples were collected from 7 German-Fleckvieh breeding bulls and were extended 1:100 rate by Triladyl extender (Minitub GmbH, Tiefenbach, Germany). Five-microliter drop of the diluted semen was placed into a Makler chamber (Sefi-Medical Instruments Ltd, Haifa, Israel) and was examined in a 20 x negative Phase Contrast trinocular (with a high resolution B/W camera) Olympus BX 40 microscope (with heated stage). Twelve microscopic field of each ejaculate were recorded on video tape and analyzed later with the above mentioned computer assisted motion analyzing systems. The non-motile %, motile %, progressive (linear movement) motile % of sperm cells and their average path velocity were examined. Mean values and Pearson correlation coefficients were determined for these data (SAS). The non-motile %, motile % and progressive motile % of sperm cells showed very high positive correlation (r=0.99, r=0.99 and r=0.98; P<0.0001), while average path velocity values did not correlated with each other. These results suggest that valuable motility data can be obtained with both systems used video recorded images. This fact looks very promising for further data (image, video tape) exchanges between research groups used different computer assisted semen motility analyzing methods.

KEY WORDS: semen, motility, analysis, CASA