IMPLANTATION AND PREGNANCY - A
Monday, August 2, 2004
10:30 AM–12:30 PM
(264) ANGIOGENESIS AND APOPTOSIS IN THE ENDOMETRIUM AND MATERNO-FETAL BOUNDARY OF IMPLANTATION SITE DURING THE MENSTRUAL CYCLE AND EARLY PREGNANCY IN RHESUS MONKEY.
Wei, Peng1, Jin, Xuan1, Hu, Zhao-Yuan1, Han, Chun-Sheng1, Liu, Yi-Xun1, 1 State Key Laboratory of Reproductive Biology, Beijing, P.R.China
ABSTRACT- Angiogenesis and apoptosis are the major phenomena in regulating turnover of the endometrium during menstruation and implantation. To study the possible role and regulation of these processes in primate endometrium, the expression of angiogenesis and apoptosis-related molecules, VEGF/bFGF and their receptors, Fas/FasL, Bcl-2, and Bax were analyzed in relation to occurrence of apoptosis and proliferation in cycling and pregnant endometrium of rhesus monkey by immunohistochemistry and Western blot. The cell apoptosis and proliferation were evaluated by means of in situ 3'-end labeling and Ki67 immunostaining, respectively. The mRNA and protein of VEGF and bFGF were detected in the glandular epithelial cells, strong expression was found in the proliferative phase, but declined in the secretory phase during the menstrual cycle, and strong expression of these factors was observed in the decidual cells. the Fas, FasL, Bcl-2 and Bax were co-localized predominantly in the epithelial cells of the endometrium. Modest Fas staining with no obvious change was detected throughout the menstrual cycle, while the levels of FasL and Bax protein in the epithelial cells increased in the secretory phase when apoptosis was most prevalent. In contrast, epithelial immunostaining for Bcl-2 was maximal during the proliferative phase and decreased in the secretory phase. The coordinated expression of VEGF/KDR, bFGF/Flg systems and the Fas/FasL, Bcl-2 and Bax in the cycling and pregnant endometrium of rhesus monkey suggests that the changes in endometrial growth and regression for the establishment of implantation window and the process of implantation may be regulated by the balance of these factors.
KEY WORDS: endometrium, angiogenesis, menstrual cycle, materno-fetal boundary