M277. SIGNALING PATHWAYS FOR MODULATION OF MAMMALIAN SPERM MOTILITY BY GPCR AGONISTS. Schuh, Sonya¹, Carlson, Anne¹, Xie, Fang², Conti, Marco², Hille, Bertil¹, Babcock, Donner¹, ¹ University of Washington, Seattle, WA² Stanford University School of Medicine, Stanford, CA

T278. NONINVASIVE VISUALIZATION OF MOLECULAR EVENTS IN THE MAMMALIAN ZYGOTE. Yamagata, Kazuo¹, Yamazaki, Taiga¹, Yamashita, Misuzu¹, Hara, Yuki¹, Ogonuki, Narumi², Ogura, Atsuo², ¹ University of Tsukuba, Tsukuba-city, Ibaraki, Japan² RIKEN Bioresource Center, Tsukuba-city, Ibaraki, Japan

W279. THE EFFECTS OF NITRIC OXIDE INHIBITION ON PREIMPLANTATION EMBRYO CHECKPOINT GENE REGULATION. Hendrickson, Simone¹, Steuerwald, Nury^{1, 2}, Huet-Hudson, Yvette¹, ¹ UNC Charlotte, Charlotte, NC, United States² ART Institute of New York & New Jersey, West Orange, NJ, United States

M280. DEVELOPMENT AND DNA DAMAGE IN PARTHENOGENETICALLY ACTIVATED BOVINE EMBRYOS PRODUCED FROM OOCYTES DERIVED FROM SMALL AND LARGE FOLLICLES. Bastos, Guilherme^{1, 2}, Gonçalves, Paulo Bayard¹, Bordignon, Vilceu², ¹ Federal University of Santa Maria, Santa Maria, RS, Brazil² McGill University, Ste-Anne-De-Bellevue, QC, Canada

T281. ABSENCE OF ACCESSORY SEX GLAND SECRETION PERTURBS THE ACTIVE DNA DEMETHYLATION OF SPERM GENOME IN VIVO IN FERTILIZED OOCYTES OF MOUSE AND GOLDEN HAMSTER. Poon, Hong-Kit¹, O, Wai-Sum², Ferguson-Smith, Anne³, Chow, Pat-Ham¹, ¹ The Chinese Unversity of Hong Kong, Hong Kong SAR² Hong Kong University, Hong Kong SAR³ University of Cambridge, Cambridge, UK

W282. IN VITRO DEVELOPMENT OF PORCINE NUCLEAR TRANSFER EMBRYOS WITH TREATMENT OF DIFFERENT INITIAL CULTURE TEMPERATURES AFTER ACTIVATION. Lai, Liangxue¹, Isom, Steven¹, Li, Rongfeng¹, Hao, Yanhong¹, Wax, David¹, Rucker, Edmund¹, Prather, Randall¹, ¹ University if Missouri-Columbia, Columbia, MO

M283. THE ORGANIC OSMOLYTES BETAINE AND PROLINE ARE TRANSPORTED BY A NOVEL SHARED TRANSPORT PATHWAY IN EARLY MOUSE EMBRYOS. Anas, Idris¹, Hammer, Mary-Anne ¹, Baltz, Jay¹, ¹ University of Ottawa and the Ottawa Health Research Institute, Ottawa, ON, Canada

T284. DEVELOPMENTAL CAPABILITY OF MOUSE PREIMPLANTATION EMBRYOS HEAT-STRESSED MATERNALLY. Perez-Crespo, Miriam¹, Fernandez-Gonzalez, Raul¹, Pericuesta, Eva¹, Ramirez, Miguel Angel¹, Moreira, Pedro Nuno¹, Rizos, Dimitrios¹, Rey, Raquel¹, Pintado, Belen¹, Gutierrez-Adan, Alfonso , ¹ Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA), Madrid, Spain

W285. CAPACITATION OF BOVINE SPERM WITH OUABAIN. Way, Amy¹, Graham, Barbara¹, ¹ Lock Haven University, Clearfield, PA

M286. OSMOTIC STRESS INDUCED p38 MAPK ACTIVATION IN PREIMPLANTATION MOUSE EMBRYOS DURING IN VITRO CULTURE. Fong, Barry^{1, 2}, Watson, Andrew^{1, 2}, ¹ University of Western Ontario, London, ON, Canada² Lawson Health Research Institute, London, ON, Canada

T287. CHANGES IN HISTONE MODIFICATION UPON THE ACTIVATION OF DORMANT MOUSE EMBRYOS. Matsuhashi, Tamako¹, Aoki, Fugaku², Sakai, Senkiti¹, ¹ University of Tokyo, Tokyo, Japan² University of Tokyo, Kashiwa, Japan

W288. FUNCTIONAL GENOMICS STUDIES OF OOCYTE AND EARLY EMBRYONIC DEVELOPMENT: POTENTIAL ASSOCIATION OF FOLLISTATIN TRANSCRIPT ABUNDANCE WITH OOCYTE COMPETENCE. Patel, Osman ¹, Bettegowda, Anilkumar ¹, Ireland, James¹, Coussens, Paul¹, Lonergan, Patrick², Smith, George¹, ¹ Michigan State University, E. Lansing, MI² University College Dublin, Dublin, Ireland

M289. SPERM ACROSOMAL SLLP2 IS INVOLVED IN BINDING TO EGG PLASMA MEMBRANE AND PERIVITELLINE MATRIX. Mandal, Arabinda¹, Digilio, Laura¹, Klotz, Kenneth¹, Flickinger, Charles¹, Herr, John¹, ¹ University of Virginia, Charlottesville, VA

T290. THE GTPases Rho, Rac, AND Cdc42 ARE PRESENT IN PORCINE SPERM AND AT LEAST ONE PLAYS A ROLE IN MEDIATING THE ZONA PELLUCIDA INDUCED ACROSOME REACTION. Ducummon, Carl^{1, 2}, Berger, Trish², ¹ Presbyterian Hospital, Dallas, TX² University of California, Davis, CA

W291. TELOMERASE ACTIVITY LEVELS IN MOUSE PREIMPLANTATION EMBRYOS. Czerwiec, Eva^{1, 2}, Liu, Lin^{1, 2}, Keefe, David^{1, 2}, ¹ Brown Medical School-Women and Infants Hospital of Rhode Island, Providence, RI² Marine Biological Laboratory, Woods Hole, MA

M292. UBIQUITIN C-TERMINAL HYDROLASE PGP9.5 IS INVOLVED IN SPERM ACROSOMAL FUNCTION AND ANTIPOLYSPERMY DEFENSE. Yi, Young-Joo¹, Manandhar, Gaurishankar¹, Sutovsky, Miriam¹, Park, Chang-Sik², Sutovsky, Peter¹, ¹ University of Missouri-Columbia, Columbia, MO² Chungnam National University, Daejeon, Korea

T293. THE EFFECTS OF A FUNCTION-BLOCKING ANTI-1 INTEGRIN SUBUNIT ANTIBODY ON SPERM-EGG BINDING ARE AFFECTED BY THE EXPERIMENTAL PARAMETERS OF THE IVF ASSAYS. Vjugina, Ulyana¹, Zhu, Xiaoling¹, Evans, Janice¹, ¹ Johns Hopkins University, School of Public Health, Baltimore, MD

W294. GENOME REPROGRAMMING INVOLVES ERASING CELL MEMORY DURING MEIOSIS. Akiyama, Tomohiko¹, Liu, Honglin¹, Kim, Jin-Moon¹, Nagata, Masao¹, Aoki, Fugaku¹, ¹ University of Tokyo, Kashiwa, Japan

M295. FEEDER LAYER- AND SERUM-FREE GROWTH OF RHESUS MONKEY EMBRYONIC STEM CELLS. Zhang, Xiuzhen^{1, 2}, Li, Tianqing ^{1, 2}, Wang, Shufen^{1, 2}, Xie, Yunhua ¹, Ji, Weizhi¹, ¹ The Chinese Academy of Sciences, Kunming Institute of Zoology, Kunmingnnan, Yunnan, P.R. China² The Chinese Academy of Sciences, Beijing, P.R. China

T296. POSSIBLE ROLE OF A POLY(A) POLYMERASE, MOUSE GLD-2, IN OOCYTE MATURATION. Nakanishi, Tomoko¹, Kubota, Haruka¹, Ishibashi, Naoko¹, Kumagai, Satoshi¹, Watanabe, Hiromi¹, Yamashita, Misuzu¹, Kashiwabara, Shin-ichi¹, Miyado, Kenji², Baba, Tadashi¹, ¹ University of Tsukuba, Ibaraki, Japan² National Research Institute for Child Health and Development, Tokyo, Japan

W297. RELATIONSHIP BETWEEN PROTEASOME ACTIVITY, PROTEIN PHOSPHORYLATION AND THE ACROSOME REACTION IN HUMAN SPERM. Kong, Milene¹, Morales, Patricio¹, Diaz, Silvina¹, ¹ University of Antofagasta, Antofagasta, Chile

M298. OXIDATIVE STRESS IN SPERM DOES NOT AFFECT FERTILIZING CAPACITY BUT DISTURBS SUBSEQUENT EMBRYO DEVELOPMENT. Silva, Patricia F¹, Roelen, Bernard A¹, Colenbrander, Ben¹, Gadella, Bart¹, ¹ Utrecht University, Utrecht, The Netherlands

T299. DEVELOPMENTAL COMPETENCE OF IN VITRO AND IN VIVO MATURED OOCYTES: MOUSE MODEL STUDY. Wang, Yue¹, Chian, Ri-Cheng¹, ¹ McGill University, Montreal, QC, Canada

W300. INTEGRITY OF MOUSE PLACENTA IN PARTHENOGENESIS. Kawahara, Manabu^{1, 2}, Kumagai, Takuya¹, Obata, Yayoi^{1, 2}, Kono, Tomohiro^{1, 2}, ¹ Tokyo University of Agriculture, Tokyo, Japan² Bio-oriented Technology Research Advancement Institution (BRAIN), Tokyo, Japan

M301. THE BENEFICIAL EFFECTS OF INSULIN AND METFORMIN ON IN VITRO DEVELOPMENTAL POTENTIAL OF PORCINE OOCYTES AND EMBRYOS. Lee, Myeong Seop¹, Hossein, M. Shamim^{1, 2}, Kang, Sung Keun^{1, 2}, Lee, Byeong Chun^{1, 2}, Hwang, Woo Suk^{1, 2}, ¹ Seoul National University, Seoul, Korea² Seoul National University Hospital, Seoul, Korea

T302. ANALYSIS OF mRNA EXPRESSION OF PUTATIVELY IMPRINTED GENES IN BOVINE BLASTOCYSTS PRODUCED IN VIVO, PARTHENOGENETICALLY AND ANDROGENETICALLY. Ruddock, Nancy^{1, 2}, Wilson, Katrina^{1, 2}, Cooney, Melissa^{1, 2}, French, Andrew^{1, 2}, Lagutina, Irina ³, Galli, Cesare³, Holland, Michael^{1, 2}, ¹ Monash Institute of Medical Research; Monash University, Clayton, VIC, Australia² Co-operative Research Centre for Innovative Dairy Products, Melbourne, VIC, Australia³ Laboratorio di Tecnologie della Priproduzione, Cremona, Italy

W303. EFFECTS OF GLUCOSE OR FRUCTOSE AND METABOLIC REGULATORS (DNP, PES, CERULENIN) ON BOVINE EMBRYO DEVELOPMENT AND LIPID ACCUMULATION. Barcelo-Fimbres, Moises¹, Seidel, George¹, ¹ Colorado State University, Fort Collins, Colorado

M304. EFFECT OF POTASSIUM SIMPLEX OPTIMIZATION MEDIUM AND NCSU23 SUPPLEMENTED WITH BETA MERCAPTOETHANOL AND AMINO ACIDS OF IN VITRO FERTILIZED PORCINE EMBRYOS. Hashem, Md. Abul¹, Hossain , M.S.¹, Jeong , Y. W.¹, Sue , K¹, Kim , J.H¹, Lee , S.H.¹, Koo , O.J.¹, Park , S.M.¹, Lee , E.G.¹, Kang , S.K¹, Lee , B.C.¹, Hwang , W.S.¹, ¹ Seoul National University, Seoul, South Korea

T305. NITRIC OXIDE SYNTHASE ISOFORMS (NOS) ARE DIFFERENTIALLY EXPRESSED IN THE BOVINE OVIDUCT DURING THE ESTROUS CYCLE. Lapointe, Jerome¹, St-Pierre, Isabelle¹, Bilodeau, Jean-Francois¹, ¹ Centre de Recherche en Biologie de la Reproduction, Centre Hospitalier de l’Universite Laval, Quebec, QC, Canada

W306. Na⁺/H⁺ EXCHANGE DURING OOCYTE MATURATION AND FOLLOWING FERTILIZATION IN MOUSE. FitzHarris, Greg¹, Baltz, Jay¹, ¹ University of Ottawa, Ottawa, ON, Canada

M307. GLOBAL DNA METHYLATION IN PORCINE CELLS EXHIBITING VARIOUS DEGREES OF DIFFERENTIATION. Lee, Kiho¹, Fodor, William², Machaty, Zoltan¹, ¹ Purdue University, West Lafayette, IN² University of Connecticut, Storrs, CT

T308. IDENTIFYING SELECTIVE INHIBITORS OF GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE-S, A SPERM-SPECIFIC GLYCOLYTIC ENZYME. Danshina, Polina¹, Temple, Brenda¹, Rojas, Rafael¹, O'Brien, Deborah¹, ¹ University of North Carolina School of Medicine, Chapel Hill, NC

W309. DYNAMICS OF HISTONE VARIANT H2A.Z DURING PREIMPLANTATION DEVELOPMENT IN MICE. Liu, Honglin¹, Kageyama , Shun-Ichiro¹, Aoki, Fugaku ¹, ¹ University of Tokyo, Kashiwa, Japan

M310. EFFECTS OF ACROGRANIN ON BLASTOCYST HATCHING AND SUBSEQUENT ADHESION AND OUTGROWTH IN THE MOUSE. Qin, Junwen¹, Diaz-Cueto, Laura^{2, 3}, Schwarze, Juan-Enrique², Gerton, George², Imakawa, Kazuhiko ¹, ¹ The University of Tokyo, Tokyo, Japan² University of Pennsylvania Medical Center, Philadelphia, PA³ Hospital de Ginecobstetricia, Mexico D. F., Mexico

T311. EFFECT OF FIBRONECTIN (FN) ON PROTEASOMAL ACTIVITY, ACROSOME REACTION (AR), AND TYROSINE PHOSPHORYLATION IN HUMAN SPERM. Morales, Patricio¹, Diaz, Silvina¹, Kong, Milene¹, Perez, Boris¹, ¹ University of Antofagasta, Antofagasta, Chile

W312. EGG ENVELOPE GLYCOPROTEIN ZP1 INDUCES SPERM ACROSOME REACTION IN JAPANESE QUAIL (Coturnix japonica). Sasanami, Tomohiro¹, Koyanagi, Kouji, Ohtsuki, Mamoru¹, Kansaku, Norio³, Hiyama, Gen, Mori, Makoto¹, ¹ Shizuoka University, Shizuoka, Japan³ Azabu University, Sagamihara, Japan

M313. EVALUATION OF HUMAN SPERM MOTILITY AND MOVEMENT CHARACTERISTICS AFTER RECOMBINANT ZONA PELLUCIDA TREATMENT. Caballero-Campo, Pedro ¹, Chirinos, Mayel², González González-Pacheco, Mirna², Larrea, Fernando², Gerton, George¹, ¹ University of Pennsylvania Medical Center, Philadelphia, PA² Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico, D.F., Mexico

T314. IMPROVED IN VITRO EMBRYO DEVELOPMENT AND INCREASED EFFICIENCY IN PRODUCING VIABLE CALVES WITH THE OPTIMIZED DEFINED TWO STEP CULTURE MEDIA. Jang, Goo¹, Lim, Kwang Taek², Lee, Won Wou², Kim, Jong In², Ko, Kyeong Hee¹, Park, Hee Jung¹, Kim, Jung Joo¹, Kang, Sung Keun¹, Lee, Byeong Chun¹, Hwang, Woo Suk^{1, 3}, ¹ Seoul National University, College of Veterinary Medicine, Seoul, South Korea² Embyro Research Center, Seoul Dairy Corporation, Gyeonggi, South Korea³ Seoul National University, School of Agricultural Biotechnology, Suwon, South Korea

W315. CHARACTERIZATION OF THE ZONA PELLUCIDA BINDING PROTEIN (ZPBP) GENE FAMILY. Lin, Yi-Nan¹, Yan, Wei², Matzuk, Martin¹, ¹ Baylor College of Medicine, Houston, TX² University of Nevada, School of Medicine, Reno, NV

M316. FUNCTIONAL ANALYSIS OF THE DROSOPHILA NPM2 GENE. Tang, Linda¹, Lin, Haifan², Ma, Lang¹, Bellen, Hugo¹, Matzuk, Martin¹, ¹ Baylor College of Medicine, Houston, TX² Duke University Medical School, Durham, NC

T317. RELATIONSHIP BETWEEN MITOCHONDRIAL ACTIVITY, ENDOGEN HYDROGEN PEROXIDE AND APOPTOSIS IN OOCYTES, COMPETENT AND NON-COMPETENT EARLY BOVINE IN VITRO PRODUCED EMBRYOS. Tarazona, Ariel¹, Jimenez, Marlene¹, Velez, Carlos¹, Olivera-Angel, Martha¹, ¹ Universidad de Antioquia, Medellín, Antioquia, Colombia

W318. MALE MICE LACKING SPERIOLIN PRODUCE DEFECTIVE SPERM AND ABNORMAL ZYGOTES. Goto, Masuo^{1, 2}, Goulding, Eugenia², Eddy, Edward², O'Brien, Deborah¹, ¹ University of North Carolina School of Medicine, Chapel Hill, NC² National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC

M319. INVOLVEMENT OF AN OOCYTE-SPECIFIC NOVEL GENE, OOG1 , IN ZYGOTIC GENOME ACTIVATION VIA RAS SIGNALING PATHWAY. Tsukamoto, Satoshi¹, Ihara, Ryo¹, Aizawa, Akira², Imai, Hiroshi¹, Minami, Naojiro¹, ¹ Kyoto University, Kyoto, Japan² Maebashi Institute of Animal Science, Livestock Improvement Association JAPAN, Inc., Maebashi, Japan

T320. DERIVATION, MAINTENANCE AND CHARACTERIZATION OF BOVINE ES-LIKE CELLS. Vassiliev, Ivan^{1, 2}, McConnell, Helen ^{1, 2}, Verma, Paul^{1, 2}, ¹ Monash University, Clayton, Victoria, Australia² CRC for Innovative Dairy Products, Melbourne, Victoria, Australia

W321. MATERNAL STEM-LOOP BINDING PROTEIN IS DEGRADED IN 2-CELL EMBRYOS AT THE G2/M BORDER BY A CELL-CYCLE-REGULATED MECHANISM. Zhang, Wenling¹, Poirier, Luc¹, Clarke, Hugh¹, ¹ Mcgill University, Montreal, QC, Canada

M322. DIFFERENTIAL RESPONSE OF PORCINE PREIMPLANTATION EMBRYOS TO TIMING VARIATIONS OF AN EXOGENOUSLY APPLIED HEAT STRESS. Isom, S. Clay¹, Alinea, Corinne¹, Dillender, Jessica¹, Prather, Randall¹, Rucker III, Edmund¹, ¹ University of Missouri-Columbia, Columbia, MO

T323. TREATMENT OF PROGESTERONE INCREASES AKT PHOSPHORYLATION IN BOAR SPERMATOZOA. Jang, Sunphil¹, Yi, Lee S. H.¹, ¹ Sungkyunkwan University, Suwon, Korea

W324. p38 MAPK IS AN UPSTREAM REGULATOR OF p53 ACTIVATED EVENTS DURING MOUSE PREIMPLANTATION DEVELOPMENT. Hickson, Jenny^{1, 2}, Watson, Andrew^{1, 2}, ¹ University of Western Ontario, London, ON, Canada² Lawson Health Research Institute, London, ON, Canada

M325. EXPRESSION OF MYOGENIC REGULATORY FACTORS (MRFs) IN DAY 30 PIG CONCEPTUSES IS AFFECTED BY UTERINE CROWDING. Tse, Wai-Yue¹, Town, Susanna¹, Putman, Charles¹, Foxcroft, George¹, Dixon, Walter¹, ¹ University of Alberta, Edmonton, AB, Canada

T326. QUANTITATIVE ANALYSIS OF PRION GENE EXPRESSION DURING EARLY BOVINE DEVELOPMENT. Peralta, Oscar¹, Huckle, William ¹, Martinez , Mario ², Correa , Jorge ², Gatica, Renato ², Eyestone, Willard¹, ¹ Virginia-Maryland Regional College of Veterinary Medicine, Blacksburg, VA² Austral University, Valdivia, Chile

W327. EFFECT OF VASCULAR ENDOTHELIAL GROWTH FACTOR ON THE DEVELOPMENT OF OVINE EMBRYOS IN VITRO. Luo, Hailing¹, Tian, Shujun¹, Zhu, Shien¹, ¹ China Agricultural University, Beijing, P.R.China

M328. RELATIONSHIPS BETWEEN SPERM MORPHOLOGY AND IN VITRO FERTILIZATION ABILITY IN MICE. Kawai, Yasuhiro¹, Hata, Tomoko¹, Suzuki, Osamu¹, Matsuda, Junichiro¹, ¹ National Institute of Infectous Diseases, Tokyo, Japan

T329. INFLUENCE OF INTEGRINS (_v AND ₅) IN BOVINE SPERM-EGG BINDING, AND FERTILIZATION IN VITRO. Goncalves, Roseli¹, Killian, Gary¹, ¹ The John O. Almquist Research Center, The Pennsylvania State University, University Park, PA

W330. DNA METHYLATION PROFILES OF DONOR CELLS AND FETAL TISSUES OF BOVINE CLONES. Kremenskoy, Maksym¹, Kremenska, Yuliya¹, Hattory, Naka¹, Tanaka, Satoshi¹, Hasizume, Kazuyoshi², Shiota, Kunio¹, ¹ The University of Tokyo, Tokyo, Japan² Iwate University, Morioka, Japan

M331. TOPOISOMERASE II AND ASSOCIATED NUCLEASE ACTIVITY IN MATURE MOUSE SPERMATOZOA. Shaman, Jeffrey ¹, Prisztoka, Renata¹, Ward, W. Steven¹, ¹ University of Hawaii, Honolulu, HI

T332. EFFECT OF OXYGEN TENSION ON HEAT-SHOCK INDUCED APOPTOSIS AND DEVELOPMENT OF PREIMPLANTATION EMBRYOS. de Castro e Paula, Luiz ¹, Hansen, Peter¹, ¹ University of Florida, Gainesville, FL

W333. THE EFFECT OF ACROSOME ON ICSI OUTCOME. Morozumi, Kazuto¹, Yanagimachi, Ryuzo¹, ¹ University of Hawaii Medical School, Honolulu, Hawaii

M334. ANALYSIS OF PROTEIN GLYCOSYLATION IN A SPERM-SOMATIC CELL ADHESION SYSTEM. Clark, Gary¹, Khoo, Kay-Hooi ², Wong, Nyet ³, Sutton-Smith, Mark³, Patankar, Manish⁴, Lattanzio, Frank¹, Morris, Howard^{3, 5}, Dell, Anne³, ¹ Eastern Virginia Medical School, Norfolk, VA² National Taiwan University, Taipei, Taiwan R.O.C.³ Imperial College London, London, UK⁴ University of Wisconsin-Madison, Madison, WI⁵ M-SCAN, Ascot, UK

T335. NUCLEAR LOCALIZATION IN BOVINE EMBRYOS OF THE TRANSCRIPTION FACTORS TBP AND HMGB2 DURING THE MATERNAL TO ZYGOTIC TRANSITION. Vigneault, Christian¹, McGraw, Serge¹, Roy, Gabrielle¹, Sirard, Marc-André¹, ¹ Université Laval, Québec, QC, Canada

W336. CALPAIN PARTICIPATION IN CAPACITATION-ACROSOMAL REACTION IN GUINEA PIG SPERMATOZOA. Bastian, Yadira ¹, Hernandez-Gonzalez, Enrique Oton¹, Mujica , Adela¹, ¹ Cinvestav-IPN, Mexico D.F., D.F., Mexico

M337. DEVELOPMENTAL AND FUNCTIONAL EVIDENCE OF NUCLEAR IMMATURITY IN PREPUBERTAL SHEEP OOCYTES. Ptak, Grazyna¹, Matsukawa, Kazutsugu¹, Palmieri, Chiara¹, Della Salda, Leonardo¹, Loi, Pasqualino¹, ¹ University of Teramo, Teramo, Italy

T338. INVOLVEMENT OF NITRIC OXIDE IN THE PHOSPHORYLATION OF MEK-LIKE AND THR-GLU-TYR MOTIFS DURING HUMAN SPERM CAPACITATION. O'Flaherty, Cristian¹, de Lamirande, Eve¹, Gagnon, Claude¹, ¹ Royal Victoria Hospital-McGill University, Montreal, QC, CANADA

W339. ACTIVATION OF PROTEIN KINASE C AND SUBSEQUENT INHIBITION OF PROTEIN PHOSPHORYLATION IMPROVE EMBRYONIC DEVELOPMENT OF BOVINE OOCYTES AFTER ICSI. Suh, Tae Kwang¹, Seidel, George¹, ¹ Colorado State University, Fort Collins, CO

M340. CHARACTERIZATION OF TRYPSIN-LIKE PROTEOLYTIC ACTIVITY FROM THE THELYCUM OF THE Penaeus monodon FEMALE. Kruevaisayawan, Hathairat^{1, 2}, Vanichviriyakit, Rapeepun^{1, 2}, Murphy, Melinda ³, Hennebold, Jon³, Basak, Ajoy¹, Weerachartyanukul, Wattana², Sobhon, Prasert², Tanphaichitr, Nongnuj^{1, 4}, ¹ Ottawa Health Research Institute, Ottawa, ON, Canada² Mahidol University, Bangkok, Thailand³ Oregon Health & Science University, Beaverton, OR⁴ University of Ottawa, Ottawa, ON, Canada

T341. ESTABLISHMENT OF TROPHOBLASTIC STEM LIKE CELL FROM MOUSE PARTHENOGENETIC EMBRYOS. Ogawa, Hihideko^{1, 2}, Shikanai, Mima¹, Kumagai, Takuya¹, Usami, Yusuke¹, Tanaka, Satoshi³, Arima, Hirotaka⁴, Kono, Tomohiro^{1, 2}, ¹ Tokyo Unversity of Agriculture, Tokyo, Japan² Bio-oriented Technology Research Advancement Institution (BRAIN), Tokyo, Japan³ The University of Tokyo, Tokyo, Japan⁴ Kyusyu University, Beppu, Japan

W342. TEMPORAL CHANGES IN GENES INVOLVED IN FOLLICULAR GROWTH AND CUMULUS OOCYTE COMPLEX DURING SUPER OVULATION IN FSH RECEPTOR HAPLOINSUFFICIENT MICE. Yang, Yinzhi¹, Aravindakshan, Jayaprakash¹, Chen, Xinlei¹, Sairam, M Ram ¹, ¹ Clinical Research Institute of Montreal, Montreal, Quebec, Canada

M343. THE EFFECT OF VITRIFICATION IN MOUSE ZYGOTES ON THE MITOTIC SPINDLE. Hassani Bafrani, Hassan¹, ¹ Kashan University of Medical Sciences, KASHAN, IRAN

T344. EPIGENETIC STUDY ON TERATOMA FORMATION. Kremenska, Yuliya¹, Kremenskoy, Maksym¹, Tanaka, Satoshi¹, Shiota, Kunio¹, ¹ The University of Tokyo, Tokyo, Japan

W345. THE ROLE OF ACTIN IN SPERM ACROSOMAL EXOCYTOSIS. French, Stephanie ¹, Cruz, Aurora ¹, Shi, Xudong ¹, Miller, David¹, ¹ University of Illnois at Urbana-Champaign, Urbana, IL

M346. WITHDRAWN. Fondong, Margaret^{1, 2}, Basak, Ajoy¹, Tanphaichitr, Nongnuj^{1, 2}, ¹ Ottawa Health Research Institute, Ottawa, ON, Canada² University of Ottawa, Ottawa, ON, Canada

T347. DETECTION OF RNA BINDING PROTEIN mRNAS AND PROTEINS IN MOUSE AND BOVINE EMBRYOS. Calder, Michele ¹, Watson, Andrew¹, ¹ University of Western Ontario, London, ON, Canada

W348. LEUKEMIA INHIBITORY FACTOR (LIF) AND ITS RECEPTOR ARE EXPRESSED AND FUNCTIONAL DURING GV TO MII IN VITRO TRANSITION OF OVINE OOCYTES. Brevini, Tiziana A¹, Matsukawa, Kazutsugu², Lopes, Federica², Cillo, Fabiana¹, Antonini, Stefania¹, Gandolfi, Fulvio ¹, Loi, Pasqualino², Ptak, Grazyna², ¹ University of Milan, Milan, Italy² University of Teramo, Teramo, Italy

M349. ASSESSMENT OF PRONUCLEAR FORMATION FOLLOWING IVF WITH BOVINE SPERMATOZOA HAVING ABNORMAL MORPHOLOGY AFTER THERMAL INSULATION OF THE TESTIS. Walters, Anneke ¹, Saacke, Richard¹, Pearson, Ronald¹, Gwazdauskas, Francis¹, ¹ Virginia Polytechnic Institute and State University, Blacksburg, VA

T350. THE DISTRIBUTION OF POLYMERIZED ACTIN AND NUCLEI WHEN HAMSTER BLASTOCYSTS EXPRESS TROPHECTODERM PROJECTIONS IN UTERO. Harris, Keith¹, Calderon, Jesus¹, Gonzales, David¹, ¹ Arizona State University West, Glendale, Arizona

W351. HIGH PROTEIN DIET AFFECTS THE PROTEOMIC PROFILE OF IN-VIVO DEVELOPED MOUSE BLASTOCYSTS. Katz-Jaffe, Mandy¹, Linck, Donald ¹, Blakesley, Jesse¹, Schoolcraft, William¹, Gardner, David¹, ¹ Colorado Center for Reproductive Medicine, Englewood, Colorado

M352. ACETYLCHOLINE-INDUCED CA²⁺ TRANSIENT AND CA²⁺ CURRENT IN MOUSE EARLY EMBRYOS. Kang, Dawon¹, Yoon, Sook-Young², Hong, Seong-Geun¹, Han, Jaehee¹, ¹ Gyeongsang National University, Jinju, South Korea² University of Massachusetts, Amherst, MA

T353. SYNTHESIS OF TUMOR NECROSIS FACTOR-STIMULATED GENE-6 PROTEIN BY CUMULUS AND GRANULOSA CELLS IN PORCINE PREOVULATORY FOLLICLE. Nagyova, Eva¹, Camaioni, Antonella², Prochazka, Radek ¹, Day, Anthony ³, Salustri, Antonietta², ¹ Academy of Sciences of the Czech Republic, Libechov, Czech Republic² University of Rome Tor Vergata, Rome, Italy³ University of Oxford, Oxford, United Kingdom

W354. EFFECT OF THE KINDS OF GENES USING FOR TRANSFECTION ON IN VITRO DEVELOPMENT OF TRANSFECTED SOMATIC CELL NUCLEAR TRANSFERRED EMBRYOS IN CATTLE. Yang, ByoungChul¹, Kim, Dong Hoon¹, Kim, Se Woong¹, Lee, Poong Yeon¹, Lee, Yen Keun¹, Park, Hyo Suck¹, Yang, Boh Suk ¹, Chang, Won Kyong ¹, ¹ National Livestock Research Institute, RDA, Suwon, Republic of Korea

M355. EFFECT OF PORCINE FOLLICULAR FLUID PROTEIN FRACTIONS ON MATURATION, IN VITRO FERTILIZATION, AND POLYSPERMY. Ducolomb, Yvonne¹, Gonzalez-Marquez, Humberto¹, Fierro, Reyna¹, Romo, Salvador², Betancourt, Miguel¹, ¹ Universidad Autonoma Metropolitana- Iztapalapa, Mexico, DF, Mexico² Universidad Nacional Autonoma de Mexico, Cuautitlan, Estado de Mexico, Mexico

T356. IDENTIFICATION OF AN OOCYTE-SPECIFIC OXYSTEROL BINDING PROTEIN IN RAINBOW TROUT. Ramachandra, Raghuveer¹, Yao, Jianbo¹, ¹ West Virginia University, Morgantown, WV

W357. EFFECTS OF GLUCOSE, L-LACTATE, AND PYRUVATE IN CULTURE MEDIA ON EMBRYONIC DEVELOPMENT AND METABOLISM OF IVF-DERIVED FELINE EMBRYOS RELATIVE TO EMBRYOS GROWN IN VIVO. Herrick, Jason ¹, Bond, Jennifer¹, Magarey, Genevieve¹, Bateman, Helen¹, Krisher, Rebecca², Dunford, Susan³, Swanson, William¹, ¹ Cincinnati Zoo and Botanical Garden, Cincinnati, OH² Purdue University, West Lafayette, IN³ University of Cincinnati, Cincinnati, OH