W663. DEVELOPMENT OF MOUSE TETRAPLOID SEMI-CLONED EMBRYOS IN VITRO AND IN VIVO. Wang, Eryao^{1, 2}, Ding, Chenhui¹, Chen, Xinjie, Yu, Yang¹, Li, Xuemei¹, Jiao, Lihong¹, Wang, Liu¹, Ji, Weizhi², Zhou, Qi¹, ¹ Chinese Academy of Sciences, The State Key Laboratory of Reproduction Biology Institute of Zoology, Beijing, PR China² Chinese Academy of Sciences, Kunming Institute of Zoology, Kunming, Yunnan, PR China

M664. EFFECT OF ASCORBIC ACID 2-O--GLUCOSIDE AND ALANYL-GLUTAMINE ON IN VITRO GROWTH CULTURE OF PREANTRAL FOLLICLES FROM CRYOPRESERVED MOUSE OVARIES. Suzuki, Osamu¹, Hata, Tomoko¹, Takekawa, Naho¹, Koura, Minako¹, Takano, Kaoru¹, Yamamoto, Yoshie¹, Noguchi, Yoko¹, Uchio-Yamada, Kozue¹, Matsuda, Junichiro¹, ¹ National Insititute of Infectious Diseases, Tokyo, Japan

T665. EFFECTS OF CRYOPROTECTANTS ON ALPACAS SPERMATOZOA DURING COOLING PROCESS. Valdivia, Martha¹, Canorio, Nadia¹, Carrillo, Eva¹, Uipan, Pedro¹, ¹ Universidad Nacional Mayor de San Marcos, Lima, Peru

W666. VITRIFICATION READY FOR REPRODUCTIVE MEDICINE: BLASTOCYST VITRIFICATION VERSUS CONVENTIONAL CRYOPRESERVATION. Liebermann, Juergen¹, Knopoff, Elissa¹, Matthews, Jill¹, Erman, Amanda¹, Tucker, Michael^{1, 2}, ¹ Fertility Centers of Illinois, Chicago, IL² Georgia Reproductive Specialists, Atlanta, GA

M667. NUMBER OF ANTRAL FOLLICLES DURING FOLLICULAR WAVES IS VARIABLE AMONG BEEF CATTLE, HIGHLY REPEATABLE WITHIN INDIVIDUALS, AND POSITIVLY ASSOCIATED WITH SUPEROVULATION RESPONSE AND EMBRYO YIELD. Ward, Fabian¹, Lonergan, Patrick¹, Ireland, James², Evans, Alexander C¹, ¹ University College Dublin, Dublin, Ireland² Michigan State University, East Lansing, MI

T668. APOPTOSIS IN MOUSE PREIMPLANTATION EMBRYOS PRODUCED IN THE DIFFERENT SYSTEMS. Bing, Yin Zhong ¹, Chian, Ri Cheng¹, Tan, Seang Lin¹, Zhao, Xin¹, ¹ McGill University, Montreal, QC, Canada

W669. EFFECTS OF OSMOTIC CHANGES AND CRYOPROTECTANT TOXICITY ON MOUSE OOCYTE DEVELOPMENTAL CAPACITY AFTER IN VITRO FERTILIZATION. Huang, Jack Y¹, Chian, Ri-Cheng¹, ¹ McGill University, Montreal, QC, Canada

M670. EFFECT OF FSH STARVATION ON THE DEVELOPMENTAL COMPETENCE OF BOVINE OOCYTES IN VITRO. Durairaj, Saravanan¹, Mayes, Mario¹, Adams, Gregg¹, Singh, Jaswant¹, ¹ Western College of Veterinary Medicine, Saskatoon, SK, Canada

T671. THE OCCURRENCE OF PHOSPHORYLATED HISTONE H2A.X IN PORCINE EMBRYOS PRODUCED BY SOMATIC CELL NUCLEAR TRANSFER AND PARTHENOGENETIC ACTIVATION. Che, Limei¹, Lalonde, Annie¹, Bordignon, Vilceu¹, ¹ McGill University, Ste-Anne-De-Bellevue, QC, Canada

W672. NaK-ATPASE AS A SIGNALING MOLECULE DURING BOVINE SPERM CAPACITATION. Thundathil, Jacob¹, Baines, Shaun¹, Hickey, Katie¹, Anzar, Muhammad¹, Buhr, Mary¹, ¹ University of Guelph, Guelph, ON, Canada

M673. EFFECT OF TESE MEDIUM ON THE MOTILITY OF SPERMATOZOA FROM EJACULATE, EPIDIDYMIS AND TESTIS IN HUMAN. Zheng, L. ¹, Feng, H.¹, ¹ Reproductive Biology, ART and Solution, New Hyde Park, NY

T674. MORPHOLOGICAL STATUS OF ACTIVE MITOCHONDRIA IN VITRIFIED BOVINE GV OOCYTES AFFECTS SUBSEQUENT DEVELOPMENT. Abe, Yasuyuki¹, Aono, Nobuya², Hara, Kenshiro, Matsumoto, Hiromichi¹, Sasada, Hiroshi¹, Sato, Eimei¹, ¹ Tohoku University, Sendai, Japan² Yoshida Lady’s Clinic, Sendai, Japan

W675. A COMPARIOSN OF IFN- MESSENGER RNA EXPRESSION BETWEEN SOMATIC NUCLEAR TRANSFER AND IN VITRO PRODUCED FEMALE BOVINE BLASTOCYSTS. Kimura, Koji¹, Akagi, Satoshi², Takahashi, Seiya², Iwata, Hisataka³, Kadokawa, Hiroya¹, Aoki, Mari⁴, Hirako, Makoto⁵, ¹ National Institute of Livestock and Grassland Science, Reproductive Physiology Lab, Nasushiobara, Tochigi, Japan² National Institute of Livestock and Grassland Science, Reproductive Cell Biology Lab, Tsukuba, Ibaraki, Japan³ Tokyo University of Agriculture, Atsugi, Kanagawa, Japan⁴ National Agriculture Research Center for Hokkaido Region, Sapporo, Hokkaido, Japan⁵ National Agriculture and Bio-oriented Research Organaization, Tsukuba, Ibaraki, Japan

M676. CHRONIC SURGERY COLLECTION OF SUPEROVULATED OOCYTES FROM MOUSE. Wang, Min-Kang¹, Zhang, Tian¹, ¹ Yunnan Normal University, Kunming, Yunnan, PR. China

T677. IMPROVEMENT OF THE FREEZE-DRYING METHOD FOR MOUSE SPERMATOZOA. Kawase, Yosuke¹, Hani, Toshio¹, Kamada, Nobuo¹, Jishage, Kou-ichi¹, Suzuki, Hiroshi^{2, 3}, ¹ Chugai Research Institute for Medical Science, Inc., Gotemba, Japan² National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan³ The University of Tokyo Graduate School of Medicine, Bunkyo-ku, Japan

W678. GnRH-INDUCED OVULATION IN HEIFERS: EFFECTS OF STAGE OF FOLLICULAR WAVE. Atkins, Jacqueline¹, Busch, Daniel¹, Bader, Jonathan¹, Schafer, Daniel¹, Lucy, Matthew¹, Patterson, David¹, Smith, Michael¹, ¹ University of Missouri-Columbia, Columbia, MO

M679. REPROGRAMMING OF LAMIN A/C FOLLOWING CROSS-SPECIES (PRIMATE FIBROBLAST TO BOVINE OOCYTE) NUCLEAR TRANSFER. Byrne, James¹, Mitalipov, Shoukhrat¹, Sparman, Michelle¹, Ramsey, Cathy¹, Wolf, Don^{1, 2}, ¹ Oregon National Primate Research Center, Beaverton, OR² Oregon Health & Science University, Portland, OR

T680. CLEAVAGE OF RAT CLONE EMBRYOS BY SOMATIC NUCLEAR TRANSFER USING FETAL FIBROBLAST CELLS REPROGRAMMED BY XENOPUS EGG EXTRACTS. Tomioka, Ikuo¹, Sugawara, Atsushi¹, Shinozawa, Tadahiro¹, Inai, Kentaro¹, Sasada, Hiroshi¹, Sato, Eimei¹, ¹ Tohoku University, Sendai, Japan

W681. EFFECTS OF UNDERNUTRITION ON IN VITRO FERTILIZATION (IVF) AND EARLY EMBRYONIC DEVELOPMENT IN SHEEP. Grazul-Bilska, Anna¹, Borowczyk, Ewa ¹, Caton, Joel¹, Bilski, Jerzy ¹, Weigl, Robert. ¹, Kirsch, James ¹, Vonnahme, Kimberly ¹, Borowicz, Pawel ¹, Reynolds, Lawrence ¹, Redmer, Dale ¹, ¹ North Dakota State University, Fargo, ND

M682. PRODUCTION OF SALMON THAT GENERATES ONLY TROUT OFFSPRING; USE OF STERILIZED TRIPLOID AS A RECIPIENT. Okutsu, Tomoyuki¹, Yoshizaki, Goro^{1, 2}, Takeuchi, Toshio¹, ¹ Tokyo University of Marine Science and Technology, Minato-ku, Tokyo, Japan² PRESTO, Japan Science and Technology Corporation, Kawaguchi-shi, Saitama, Japan

T683. 4-VINYLCYCLOHEXENE DIEPOXIDE-INDUCED PRIMORDIAL FOLLICLE DEPLETION IN CANINE OVARIES. Miers, Heidi¹, Dyer, Cheryl¹, Craig, Royce ², Kalleco, Doreen¹, Karr, Katie¹, Robinson, Zachary ¹, Marion, Samuel³, Hoyer, Patricia³, Balok, Clinton², Mayer, Loretta¹, ¹ Northern Arizona University, Flagstaff, AZ² Crownpoint Institute of Technology, Crownpoint, NM³ University of Arizona, Tucson, AZ

W684. DNA FRAGMENT AND APOPTOSIS CAUSED BY MICROMANIPULATION IN MOUSE SOMATIC CELL NUCLEAR TRANSFER. Ding, Chenhui^{1, 2}, Chen, Xinjie¹, Yu, Yang¹, Wang, Eryao¹, Li, Xuemei¹, Jiao, Lihong¹, Wang, Liu¹, Ji, Weizhi², Zhou, Qi¹, ¹ Chinese Academy of Sciences, The State Key Laboratory of Reproduction Biology Institute of Zoology, Beijing, PR China² Chinese Academy of Sciences, Kunming Institute of Zoology, Kunming, Yunnan, PR China

M685. LOW O₂ TENSIONS AND THE PREVENTION OF DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS. Ezashi, Toshihiko¹, Das, Padmalaya¹, Roberts, R. Michael¹, ¹ University of Missouri, Columbia, MO

T686. SUCCESSFUL TRANSFER OF MOUSE EMBRYONIC NUCLEI INTO ENUCLEATED ZYGOTES. Greda, Pawel¹, Karasiewicz, Jolanta¹, Modlinski, Jacek¹, ¹ Polish Academy of Sciences, Jastrzebiec, Poland

W687. LIVE BIRTH RATE IN MICE AFTER ELECTROFUSING CUMULUS CELL WITH ENUCLEATED OOCYTE. Yu, Yang¹, Wang, Eryao¹, Li, Xuemei¹, Zhao, Chunli¹, Ding, Chenhui¹, Jiao, Lihong¹, Wang, Liu¹, Zhou, Qi¹, ¹ Institute of Zoology, Chinese Academy of Sciences, Beijing, PR China

M688. THE DEVELOPMENTAL POTENTIAL OF TRANSFERRED FERRET EMBRYOS IS DEPENDENT UPON THE REPRODUCTIVE HISTORY OF THE RECIPIENT FEMALE. Li, Ziyi¹, Sun, Xingshen¹, Chen, Juan¹, Leno, Gregory¹, Engelhardt, John¹, ¹ University of Iowa, Iowa City, IA

T689. EXPOSURE OF C57BL/6J MALE MICE TO ELECTRIC FIELD IMPROVES COPULATION RATES WITH SUPEROVULATED FEMALES. Hori, Takuya ^{1, 2}, Yamsaard, Thicomeporn ¹, Ueta, Yoshiko Yanagimoto¹, Harakawa, Shinji ^{1, 2}, Kaneko, Etsushi ¹, Miyamoto, Akio ¹, Xuan, Xuenan ¹, Toyoda, Yutaka¹, Suzuki, Hiroshi ^{1, 3}, ¹ Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan² Hakuju Institute for Health Science, Tokyo, Japan³ The University of Tokyo, Tokyo, Japan

W690. SUSCEPTIBILITY OF BOVINE SPERM TO THE IN VITRO INDUCED ACROSOME REACTION. Birck, Anders¹, Moeller Petersen, Morten¹, Christensen, Preben¹, ¹ The Royal Veterinary and Agricultural University, Frederiksberg, Copenhagen, Denmark

M691. WITHDRAWN.

T692. A SIMPLE MODIFIED ICSI TECHNIQUE RESULTING IN HIGH PREGNANCY RATES IN MERINO SHEEP(Ovis Aries). Shin, Taeyoung ¹, Long, Charles¹, Foxworth, Gabriella¹, Hanna, Carol¹, Menges, Suzanne¹, Bormann, Christy¹, Almeida-Porada, Graca², Porada, Christopher², Glimp, Hudson², Millsap, Laura², Westhusin, Mark¹, Kraemer, Duane¹, ¹ Texas A&M University, College Station, TX² University of Nevada, Reno, NV

W693. STORAGE OF CAMEL (Camelus dromedarius) EPIDIDYMAL SPERMATOZOA IN DIFFERENT EXTENDERS AND AT DIFFERENT TEMPERATURES. Wani, Nisar¹, Nowshari, Manzoor¹, Wernery, Ulrich¹, ¹ Central Veterinary Research Laboratory, Dubai, United Arab Emirates

M694. REDUCING ISCHEMIC DAMAGE IN OVARIAN XENOGRAFTS. Israely, Tomer¹, Nevo, Nava¹, Harmelin, Alon¹, Neeman, Michal¹, Tsafriri, Alex¹, ¹ The Weizmann Institute of Science, Rehovot, Israel

T695. METHYLATION STATUS OF PUTATIVE DMRs OF PORCINE IGF2 AND H19. Han, Dong Wook¹, Im, Young Bin¹, Gupta, Mukesh Kumar¹, Uhm, Sang Jun¹, Lee, Hoon Taek¹, ¹ Konkuk University, Seoul, Korea

W696. IMPROVEMENT IN RECIPIENT MOUSE FERTILITY OF SPERMATOGONIAL TRANSPLANTATION. Ogawa, Takehiko ¹, Tamura, Yoichi¹, Kita, Kaoru¹, Kubota, Yoshinobu¹, ¹ Yokohama City University Graduate School of Medicine, Yokohama, Japan

M697. EFFECTIVENESS OF ADMINISTRATION OF GONADOTROPIN RELEASING HORMONE AT DAY 11 OR 14 POST-OVULATION FOR INCREASING FERTILITY OF LACTATING DAIRY COWS AND NON-LACTATING HEIFERS. Franco, Moises¹, Block, Jeremy¹, Jousan, Frank¹, de Castro e Paula, Luis¹, Brad, Amber¹, Hansen, Peter¹, ¹ University of Florida, Gainesville, FL

T698. GENERATION OF NORMAL PROGENY FROM CLONED MICE WITH POSTNATAL LETHALITY BY INTRACYTOPLASMIC SPERM INJECTION AFTER TESTICULAR TISSUE GRAFTING. Ohta, Hiroshi¹, Wakayama, Teruhiko¹, ¹ RIKEN Kobe Institute, Hyogo, Japan

W699. IDENTIFICATION OF SPERM ANTIGENS AS TARGETS FOR THE DEVELOPMENT OF A CONTRACEPTIVE VACCINE AGAINST THE FOSSORIAL WATER VOLE Arvicola terrestris scherman . Grignard, Elise¹, Vernet, Patrick¹, Drevet, Joel¹, ¹ Blaise Pascal University, Aubiere, France

M700. ESTABLISHMENT AND GENETIC MANIPULATION OF NOVEL COMMON MARMOSET (Challithrix jacchus) EMBRYONIC STEM CELL LINES. Sasaki, Erika¹, Hanazawa, Kisaburo², Kurita, Ryo³, Ohnishi, Yasuyuki¹, Tamaoki, Norikazu¹, Tani, Kenzaburo³, Tanioka, Yoshikuni¹, ¹ Central Institute for Experimental Animals, Kawasaki, Kanagawa, Japan² Juntendo University, Urayasu Hospital, Urayasu, Chiba, Japan³ Kyusyu University, Medical Institute of Bioregulation, Hakata, Fukuoka, Japan

T701. LENTIVIRAL GENE TRANSFER OF NERVE GROWTH FACTOR TO THE MACAQUE OVARY IN VIVO. Zelinski-Wooten, Mary¹, Dissen, Gregory¹, ¹ Oregon Health & Science University, Beaverton, OR

W702. DIFFERENTIAL EFFECTS OF FSH AND FORSKOLIN ON PRIMARY MURINE FOLLICLES IN A THREE-DIMENSIONAL IN VITRO CULTURE. Berkholtz, Courtney¹, Woodruff, Teresa¹, Shea, Lonnie¹, ¹ Northwestern University, Evanston, IL

M703. DIFFERENCES IN PRE-PUBERTAL AND ADULT OOCYTE DEVELOPMENTAL COMPETENCE IS CORRELATED WITH OOCYTE cAMP CONTENT IN THE PIG. Bagg, Melanie¹, Nottle, Mark¹, Armstrong, David¹, Grupen, Christopher¹, ¹ The University of Adelaide, Adelaide, SA, Australia

T704. DEVELOPMENT OF PORCINE EMBRYOS PRODUCED BY NUCLEAR TRANSFER FROM SOMATIC CELLS TREATED WITH PROTEIN SYNTHESIS AND CYCLIN-DEPENDENT KINASE INHIBITORS. Lalonde, Annie¹, Che, Limei¹, Bordignon, Vilceu¹, ¹ McGill University, Ste-Anne-De-Bellevue, QC, Canada

W705. INJECTION OF VASCULAR ENDOTHELIAL GROWTH FACTOR GENE STIMULATES OVARIAN FOLLICULAR DEVELOPMENT BY PROMOTING THECAL ANGIOGENESIS IN IMMATURE FEMALE RATS. Shimizu, Takashi^{1, 2}, Iijima, Koji¹, Miyabayashi, Kanako¹, Ogawa, Yoshinori¹, Miyamoto, Akio², Sasada, Hiroshi¹, Sato, Eimei¹, ¹ Tohoku University, Sendai, Japan² Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan

M706. ACROSOMAL AND STRUCTURAL DAMAGES OCCURRING DURING CRYOPRESERVATION OF HUMAN SPERM: TIME-RELATED EVENTS? Des Rosiers, Pascal¹, Légaré, Christine¹, Leclerc, Pierre¹, Sullivan, Robert¹, ¹ Université Laval, Québec, QC, Canada

T707. OVARIAN STIMULATION OF COMMON MARMOSET (Callithrix jacchus). Hirakawa, Reiko¹, Tanioka, Yoshikuni¹, Sotomaru, Yusuke^{1, 2}, Etho, Tomoo¹, Kamioka, Mithiko³, Ooiwa, Ryo³, Shimada, Akiko³, Sasaki, Erika¹, ¹ Central Institute for Experimental Animals, Kawasaki, Japan² Hiroshima University, Hiroshima, Japan³ Japan Animal Care, Tokyo, Japan

W708. FERTILIZATION ABILITY OF MOUSE SPERMATOZOA CRYOPRESERVED FOR OVER 10 YEARS. Kaneko, Takehito¹, Yamamura, Ayako¹, Ide, Yukie¹, Ogawa, Mami¹, Yanagita, Tomoko¹, Nakagata, Naomi¹, ¹ Kumamoto University, Kumamoto, Japan

M709. REVEALING APOPTOSIS IN SMALL SAMPLES OF DNA. Campbell, Kenneth¹, Ko, Sung-Hwan¹, ¹ University of Massachusetts, Boston, MA

T710. EFFECTS OF SERUM SUPPLEMENT AND NUCLEAR TRANSFER PROTOCOL ON BLASTOCYST FORMATION AND mRNA EXPRESSION IN PREIMPLANTATION PORCINE EMBRYOS. Lee, So hyun ¹, Kim, Sue¹, Jeong, Yeon woo ¹, Kim, Ji hye ¹, Koo, Ok Jae ¹, Lee, Eu gene ¹, Park, Seon mi ¹, Hashem, MD Abu¹, Hoseein, Mohammad shamim ¹, Kang, Sung keun ^{1, 2}, Lee, Byeong-chun ^{1, 2}, Hwang, Woo suk ^{1, 2}, ¹ Seoul National University, Seoul, Korea² Seoul National University Hospital, Seoul, Korea

W711. REPEATABILITY OF 2- AND 3-WAVE PATTERNS DURING THE BOVINE ESTROUS CYCLE. Jaiswal, Rajesh¹, Singh, Jaswant¹, Marshall, Laura¹, Adams, Gregg¹, ¹ University of Saskatchewan, Saskatoon, SK, Canada

M712. ORAL PROGESTOGEN, ALTRENOGEST, PROVIDES SHORT-TERM INHIBITION OF OVARIAN ACTIVITY IN THE DOMESTIC CAT. Bauer, Rosemary^{1, 2}, Pelican, Katharine¹, Wildt, David¹, Ottinger, Mary Ann², Howard, JoGayle¹, ¹ Smithsonian's National Zoological Park, Front Royal, VA² University of Maryland, College Park, MD

T713. SPERMATOGONIAL TRANSPLANTATION IN SHEEP. Rodriguez-Sosa, Jose¹, Dobson, Howard¹, Hahnel, Ann¹, ¹ University of Guelph, Guelph, ON, Canada