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(M187) IMPACT OF TESE MEDIUM ON THE PREGNANCY RATE IN DAIRY CATTLE AFTER ARTIFICIAL INSEMINATION: AN ANIMAL MODEL.

Feng, H.¹, ¹ Reproductive Biology, ART and Solution, New Hyde Park, NY

ABSTRACT- Introduction: The objective of this study was to compare the pregnancy rate in dairy cattle after sperm washing by TCM-199 and TESE medium for artificial insemination (AI). This study will provide useful data for TESE medium for use on human intra-uterus insemination (IUI), particularly for male factor infertility. Materials and Methods: TESE (Testicular Sperm Extraction) medium containing sperm stimulators was specially designed for male factor and/or poor quality sperm with lower motility. Forty estrous dairy cattle (3-5 years old) were randomly divided into two groups. Group I (N=20) was performed AI by inseminators with a standard insemination dose (10-15 million of frozen and thawed spermatozoa), the sperm specimen was prepared by pre-warmed TESE medium + 10% FCS; Group II (N=20) was performed similar procedure for AI, however, the sperm specimen was prepared by pre-warmed TCM-199 + 10% FCS. The pregnancy rates were compared between two groups. Results: The data suggests that the use of TESE medium resulted in significantly increased pregnancy rate in group I (11/20, 55%) as compared with Group II (6/20, 30%) (P<0.01). Conclusion: It is concluded that AI could achieve a higher pregnancy rate in dairy cattle after using TESE medium to prepare sperm. This data indicates TESE medium might be beneficial for use in human IUI sperm preparation, especially for male factor infertility.

KEY WORDS: Sperm, AI, IUI, TESE