Sex Determination and Development
(W717) CHARACTERIZATION OF TRANSGENIC MICE EXPRESSING YELLOW FLUORESCENT PROTEIN UNDER THE CONTROL OF HUMAN SF1 PROMOTER SEQUENCE.
Cory, Aron1, Boyer, Alexandre1, Silversides, David1, 1 University of Montreal, St-Hyacinthe, Quebec, Canada
ABSTRACT- Steroidogenic factor 1 (SF1) has been called the master gene in steroid hormone production pathways. In order to study the regulation of SF1 promoter activity we created transgenic mice that express yellow fluorescent protein (YFP) under the control of 3.5kb human SF1 5′ flanking sequences (hSF1pYFP). Expression of fluorescence was seen in the XX and XY genital ridges starting at embryonic day 11.5 (e11.5). In the female embryonic gonad fluorescence dissapeared after e12.5 but it was again detectable on and after e16.5. In the male embryonic gonad expression of fluorescence peaked between e13.5 and e14.5 but then declined until birth. Fluorescence was observed at week of age in the gonads ofboth sexes as well as in the mature male testicle. Other areas associated with SF1 expression (Including the adrenal primordium, adrenal cortical cells, the pituatary and the hypothalimus) did not exhibit fluorescence. This may indicate that transgene expression levels are too low to detect visually or that additional regions of the SF1 promoter not found in this transgene are needed to drive expression in these cell types. Further characterization of cell types exhibition fluorescence are currently in progress.
KEY WORDS: sf1, steroidogenic, yfp