PARENT SESSION

(W183) ULTRASONIC MONITORING OF FOLLICLES AND CORPORA LUTEA DURING SYNCHRONIZATION IN SUMMER ANOESTROUS NILI RAVI BUFFALOES AND THEIR SUBSEQUENT SUPEROVULATORY RESPONSE.

Ahmad, Nasim ¹, Khan,, Muhammad Irfan-ur-Rehman ¹, Ahmad, Mudassir ¹, ¹ University of Veterinary and Animal Sciences, Lahore, Pakistan

ABSTRACT- In the first experiment, effect of GnRH (Dalmeraline; FATRO; Italy) 2 ml, given intramuscularly and injection of PGF₂ (Delmazine; FATRO; Italy) 2 ml, given intramuscularly, 9 days apart, was observed on induction of ovulation and estrus in anoestrous buffaloes during summer (n=2). Ovarian follicles and corpora lutea were monitored on every other day basis, using real time, transrectal ultrasonography. Estrus detection was carried out twice daily. The diameter of the largest follicle, on the day of administration of GnRH, averaged 9 ± 0 mm. These follicles ovulated within 48 h after injection of GnRH. Interval to estrus after injection of PGF₂ was 63 ± 11 h. In the second experiment, effect of follicle stimulating hormone (FSH-p) beginning on Day 10 of estrous cycle on follicular development in buffaloes during summer (n=2) was observed. FSH-p (Sigma; total of 40 mg) was administered intramuscularly, in divided doses, twice daily on Day 10, 11, 12 and 13. PGF₂ (2 ml) was injected on Day 13. Ovarian follicles and corpora lutea were monitored on daily basis using real time, transrectal ultrasonography. Estrus detection was carried out twice daily. Superovulatory response was measured and analyzed by comparing follicular development on Day 10, i. e. beginning of FSH-p treatment, (Before) and on Day of next estrus, (After). Superovulatory response was confirmed by determining number of corpora lutea on Day 7 after estrus. Interval to estrus after injection of PGF₂ was 37 ± 10 h. Mean number of small follicles decreased (P<0.05) ′After′ FSH-p treatment than ′ Before′ . Mean number of medium and large follicles and corpora lutea increased (P<0.05) ′ After′ FSH-p treatment than ′ Before′ . It is concluded that protocol of GnRH-PGF₂ can induce ovulation and estrus in buffaloes and reasonable superovulatory response to FSH-p can be achieved during summer when given during mid luteal phase.

KEY WORDS: synchronization, superovualtion, ultrasonography, anestrous buffalo