Sex Determination and Development
(T716) FOLLICLE-STIMULATING HORMONE INDUCES CELL PROLIFERATION IN THE OVARY OF THE CHICKEN EMBRYO.
Sanchez, Guadalupe1, Salazar, Oscar 1, Mondragon, Carmen1, Mendez, Carmen1, Pedernera, Enrique1, 1 UNAM, Mexico, DF, Mexico
ABSTRACT- Cell proliferation in the ovary of the chicken embryo reaches its maximum at 9 days of incubation (d.i.), before and after this age the proliferation rate is lower than that observed at 9 d.i.. In a previous study, it was demonstrated that hypophysectomy (Hx) suppresses the peak of cell division observed at 9 d.i.. In order to evaluate the effect of FSH on cell proliferation of the embryonic ovary, Hx embryos were treated with 2.0 IU/embryo of recombinant-human FSH (Serono). Two series of experiments were performed: a) Hx embryos were treated with FSH at 8 d.i. and evaluated at 9 d.i. by measuring BrdU incorporation; and b) Hx embryos were injected with r-h FSH at 9 d.i. and the proliferation rate was measured at 13 d.i.. Both experimental designs displayed a recovery after FSH treatment in the proliferation index of the Hx embryos ( 9 d.i.: Control 12.6 ± 0.6, Hx 10.0 ± 0.8*, Hx + FSH 13.0 ± 0.7 % of BrdU labeled cells. 13 d.i.: Control 10.3 ± 1.5, Hx 3.4 ± 0.8*, Hx + FSH 9.0 ± 1.9, % of BrdU labeled cells, * P < 0.05). More evidence about the stimulatory effect of FSH on cell proliferation was obtained in an organ culture model maintaining the chicken ovary in a serum free defined medium. Ovaries of 9-day-old chicken embryos were cultured up to 72 h in DMEM-albumax on polycarbonate membranes . The proliferation index (BrdU incorporation) was incremented by r-h FSH at 24 h and 48 h of culture compared to controls. These results demonstrated that FSH stimulates cell proliferation in the chicken ovary at 9 d.i. and a unique FSH treatment reversed the impaired cell proliferation observed in Hx embryos. FSH is a cell proliferation inductor in the chicken ovary during development. This work was supported by CONACYT 45030-Q and PAPIIT-UNAM IN214403.
KEY WORDS: chicken embryo, ovary, FSH, cell proliferation