PARENT SESSION

(T665) EFFECTS OF CRYOPROTECTANTS ON ALPACAS SPERMATOZOA DURING COOLING PROCESS.

Valdivia, Martha¹, Canorio, Nadia¹, Carrillo, Eva¹, Uipan, Pedro¹, ¹ Universidad Nacional Mayor de San Marcos, Lima, Peru

ABSTRACT- Freezing/Thawing process induce damage on the sperm membranes due to is important the adequate use of cryoprotectans agents in specific and optimal concentration for protect of spermatozoas. The objective of this study was to determine the effect of cryoprotectans on the alpaca spermatozoa during cooling process. In this study we used Yolk-citrate medium supplemented with four cryoprotectans as: Dimethyl sulfoxide (DMSO), N-N- Dimethyl-acetamide (DMA), Glycerol and Propylene glycol, in different concentrations: 0.25,0.5,0.75 and 1 M. The evaluated parameters were sperm motility and integrity of membranes by hyposmotic test were analyzed. The DMA (0,25M and 0.5M) and DMSO (0,25) showed good results because the progressive motility of these spermatozoas were similar to the initial motility when were incubated 30 minutes at 4 grades Celsius. After 1 hour and 30 minutes the progressive motility were reduced only in 50 percentage when they were at 4 grades Celsius. The integrity of membranes percentage showed that 50 percentage of spermatozoas had intact membranes. By the other way the others cryoprotectants agent showed low motility percentage and the spermatozoa showed damage on theirs membrane. Our results indicate that the possible use to DMSO and DMA as cryoprotectant agent on alpaca spermatozoas during freezing process. ICGEB Grant CRP/ PER03-01 Molecular Analysis of alpacas gametes interactions

KEY WORDS: spermatozoas, cooling, cryoprotectants agent, Alpaca